On the contrary, cells adhered to BioRoot®RCS showed no signs of any cellular protrusion. Cells adhered to UltraCal® show protrusion such as filopodia. ResultsĬells adhered to ProRoot®MTA showed an increase of F-actin like protrusions, suggesting bioactivity. 20,000 cells adhered for 24 h over discs of Biodentine™, ProRoot®MTA, BioRoot®RCS and calcium hydroxide mixed either with sodium chloride 0.9%w/v or polyethylene glycol and UltraCal® were used to evaluate cell adhesion by scanning electron microscopy and cell viability by MTT assay. Primary cells were obtained from explants of young immature premolars. This study aimed to evaluate the biological response of human apical papilla cells to different calcium hydroxide formulations and three tricalcium silicate-based materials.
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